更新时间：2023-10-26 14:42
分类
(1) yeast like colony
类酵母型菌落
(2) yeast type colony
酵母型菌落
(3) Saccharomycetes
酵母菌类
(4) Yeast-Like fungus
类酵母菌
(5) yeast colony PCR
酵母菌落PCR
1.
Recombinants were identified by yeast colony PCR, then were induced by methanol and detected by SDS-PAGE analysis.
berezovskii白介素-2(interleukin-2,IL-2)到酵母表达载体pPIC9K,SalⅠ酶切后转化毕赤酵母GS115,G418抗性筛选高拷贝克隆,再由酵母菌落PCR鉴定;阳性克隆用甲醇诱导表达,SDS-PAGE分析,结果在诱导至第6d的酵母上清浓缩液中,检测到与预测林麝IL-2分子量相接近的,分子量约为18Ku的诱导表达带;TRIzol提取表达期间的酵母总RNA,并通过RT-PCR扩增IL-2mRNA,发现表达期的重组酵母细胞中存在IL-2mRNA,而对照没有检出,表明林麝IL-2存在低水平的表达。
2.
Recombinants were identified by yeast colony PCR.
5K上,SalI线性化后转化毕赤酵母GS115,418抗性筛选高拷贝克隆,再由酵母菌落PCR鉴定;阳性克隆用甲醇诱导表达,Tricine-SDS-PAGE分析,结果在诱导第2d的酵母裂解液中检测到与预期的SMAP-29分子量接近,约3。
(6) yeast-like molds
类酵母霉菌
补充资料：黑粉病菌类